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Immunoblotting Question

BarnhallBull · 2011-04-05 12:18:40

Hi guys, doing a lab report on Western Blotting and i'm trying to find an answer to this question... Apart from Nitrocellulose, name 2 other solid phases suitable for antigen/antibody immobilisation I've searched through so many papers and the only membranes I can find used are nitrocellulose and PVDF, I'm on the point of…

05-04-2011 1:18pm

#1

BarnhallBull

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Hi guys, doing a lab report on Western Blotting and i'm trying to find an answer to this question...

Apart from Nitrocellulose, name 2 other solid phases suitable for antigen/antibody immobilisation

I've searched through so many papers and the only membranes I can find used are nitrocellulose and PVDF, I'm on the point of giving up at this stage :mad::(

Sorry if it's a basic question but if anyone can answer i'd be grateful, thanks!

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#2 07-04-2011 3:38am

Improbable

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Nylon membranes also used to be used.

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#3 10-04-2011 4:50pm

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yeah the only other two I can think of are nylon and PDVF, but sure that's your answer right there!

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