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MASS SPEC - MASS APPEARS TWICE IN SAME SPECTRUM

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  • 06-06-2012 6:12pm
    #1
    Kina
    Registered Users Posts: 22 ✭


    Hi all,

    Just made a product as part of a Research project, and after running through LC-MS, the parent ion mass has appeared twice. I personally have never seen this happen before, so have you any ideas? A dimer issue has been suggested.


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    #2
    Kevster
    Closed Accounts Posts: 2,980 ✭✭✭Kevster


    Actually, I have seen this. Before I did my PhD in cancer, I worked a few months at the Dept. of Chemistry at the Uni of York and was doing MS routinely. I saw - numerous times - ion masses that were multiple-times the parent (i.e. some even more than double). I was however working with sugar molecules (hexa- and penta-saccharides).

    Which molecules are you working with?


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    #3
    ianobrien
    Registered Users Posts: 2,755 ✭✭✭ianobrien


    Are they same using TOF? Are they same using high mass accuracy?


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    #4
    zl1whqvjs75cdy
    Closed Accounts Posts: 4,042 ✭✭✭zl1whqvjs75cdy


    It sounds like something extremely hydrophobic is lodged on your column, for example detergent. This will make your column a non homogeneous surface and will cause the double banding you have described. If you or anyone else are running samples always spin filter to remove detergent. Give the column a complete flush through for several hours but depending on the detergent the column may need to be replace. They are notoriously difficult to remove once they stick onto a column.


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    #5
    sarumite
    Registered Users Posts: 2,909 ✭✭✭sarumite


    donkeyoaty0099 wrote: »
    It sounds like something extremely hydrophobic is lodged on your column, for example detergent. This will make your column a non homogeneous surface and will cause the double banding you have described. If you or anyone else are running samples always spin filter to remove detergent. Give the column a complete flush through for several hours but depending on the detergent the column may need to be replace. They are notoriously difficult to remove once they stick onto a column.

    If you have detergents, you would probably see it in the spectra though?

    I can only speak from biological sample analysis, but the more common detergents (or detergent products) have common diagnostic ions.


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