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Calibration curve

  • 28-04-2016 1:44pm
    #1
    okman
    Registered Users Posts: 17 ✭


    Hi All - I posted this also in the chemistry and physics form. I hope this isnt breaking any rules just its something biologists might be able to help with also.

    I recently did some experiments where i had to use a p-nitrophenol to create a calibration curve in order to calculate concentrations of p-nitrophenol in an assay.

    Now I realise these calibration curves are likely a little out, and i am potentially over estimating the concentrations of p-nitrophenol in my assay.

    Is it possible however, to use the extinction coefficients found in the literature for p-nitrophenl and calculate my concentrations using this data as opposed to that of my own calibration curves?

    Cheers


Comments

  • #2
    Capt'n Midnight
    Moderators, Recreation & Hobbies Moderators, Science, Health & Environment Moderators, Technology & Internet Moderators Posts: 92,789 Mod ✭✭✭✭Capt'n Midnight


    The whole point of a calibration curve is to take into account local variations in your setup. eg: your equipment not being calibrated correctly. Ask your supervisor.

    You asked about this years ago.
    http://www.boards.ie/vbulletin/showthread.php?p=83273790


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