Biology enzyme Experiments q.question

|Maestro|

in these experiments what are all the parts for
eg. radish
onion
washing up liq.
buffer
hydrogen peroxide
heating cooling

rosa

|Maestro| wrote:

in these experiments what are all the parts for
eg. radish
onion
washing up liq.
buffer
hydrogen peroxide
heating cooling

The radish contains the enzyme (catalase)
In the DNA exprt the onion is the source of DNA
Washing up liquid in the enzyme exprts is used to calculate the rate of enyme action- the height of foam is measured, the greater the height the greater the rate of enzyme action.
In the Dna exprt washing up liguid is used to break down the cell membrane.
Buffer is used either to maintain a constant pH or, if different pH values are used, to alter pH
Hydrogen peroxide is the substrate acted on
Heating activates the enzyme, if the enzyme is boiled it is denatured.
Cooling in the DNA exprt is to make sure the DNA is not denatured too much.
Hope that helps, not sure if its all correct cos I'm just going from memory and I could be wrong about some of it.

|Maestro|

What is the optimum ph of catylase?

|Maestro|

rosa wrote:

The radish contains the enzyme (catalase)
In the DNA exprt the onion is the source of DNA
Washing up liquid in the enzyme exprts is used to calculate the rate of enyme action- the height of foam is measured, the greater the height the greater the rate of enzyme action.
In the Dna exprt washing up liguid is used to break down the cell membrane.
Buffer is used either to maintain a constant pH or, if different pH values are used, to alter pH
Hydrogen peroxide is the substrate acted on
Heating activates the enzyme, if the enzyme is boiled it is denatured.
Cooling in the DNA exprt is to make sure the DNA is not denatured too much.
Hope that helps, not sure if its all correct cos I'm just going from memory and I could be wrong about some of it.

ok thanks thats great so te radish id the enzyme source
and the hydrogen peroxide is the substrate
what is the product?

rosa

The optimum pH for catalase is about 6 or 7 I think? Don't think it differs from amylase. Not too sure abot the product, maybe oxygen? That's only a guess though!

Cherry_Pie

There is no radish experiement in my book at all!! Im a bit concerned now! Can anyone describ this experiment!

|Maestro|

Cherry_Pie wrote:

There is no radish experiement in my book at all!! Im a bit concerned now! Can anyone describ this experiment!

if you have new senior biology they use amylase solution
instead of radish(which contain catylase)

so dont worry just take note that radishes contain catylase just in case its a short Q.

rosa

I think you're only required to know one or the other for the enzyme exprts. but if they ask specifically for an animal enzyme, use amylase and catalase if they ask for a plant one.